International Journal of Homoeopathic Sciences

Objective: The aim of this study was to perform In vitro assessment of Homeopathic ingredient based toothpaste against disease causing oral pathogens. To determine the antimicrobial susceptibility of disease causing periodontal pathogens to homeopathic ingredient based toothpaste through zone of inhibition and time kill assay. Herbal toothpastes are more efficacious and safe due to containing natural actives as compared with the synthetic & chemical toothpastes. The present study aimed to evaluate the poly herbal toothpaste prepared using homeopathic medicinal plants and evaluate its efficiency in the protection of oral hygiene and prevention of dental caries by inhibition of disease causing microorganism.

Methods: The toothpaste Dabur homeopathic ingredient based toothpaste was made of Hekla Lawa extract fine ash from mount Hekla Lawa, Hamamelis Virginica Extract, Plantago Major Extract, Phytolacca decandra Extract and Calendula Officinalis Extract combined in Toothpaste formulation. The product has tested against pathogenic bacteria Streptococcus mutans using agar well diffusion method. The agar-well diffusion method was used to test the antimicrobial effect. Inhibition zones formed around toothpastes after 24 hours of incubation were measured and the data collected were statistically analysed. The time-dependent killing assay was carried out on Streptococcus mutans.

Conclusions: In vitro assessment of Homeopathic ingredient based toothpaste against disease causing oral pathogens revealed the 99.9% effectiveness of toothpaste against major cavity causing oral pathogen such as S. mutans.