International Journal of Homoeopathic Sciences

UV-visible spectrophotometry is a fundamental analytical technique widely used for the qualitative and quantitative analysis of chemical substances. This study explores the application of UV-vis spectrophotometry in determining the concentration of an analyte by measuring its absorbance at a specific wavelength. Based on the Beer-Lambert Law, the method establishes a direct relationship between absorbance and concentration. A calibration curve was constructed using standard solutions, and the concentration of unknown samples was successfully determined. The simplicity, rapidity, and cost-effectiveness of the technique make it highly suitable for routine analysis in pharmaceutical, environmental, and biochemical laboratories. The results demonstrated high accuracy and reproducibility, confirming the reliability of UV-vis spectrophotometry as a tool for spectroscopic analysis.