International Journal of Homoeopathic Sciences

In this research, we evaluated the growth-inhibiting effects of sepia officinalis and tellurium, along with their strengths against Trichophyton rubrum. The objective of the current study is to examine the drug's influence. Homeopaths have been attempting to validate the remarkable efficacy of minute doses for nearly decades. The overarching implication of this research is to demonstrate the effect of the chosen homeopathic medicine on Trichophyton rubrum. An inoculum of the fungal strain Trichophyton rubrum was suspended in 2ml of MHA broth and incubated at 37°C for one day to achieve a turbidity of 0.5 McFarland standards. The antifungal efficacy was assessed using the disc diffusion method. For this procedure, the inoculum was spread over Muller Hinton Agar medium with a sterile swab. Small circular sterile paper discs (6mm in diameter) were impregnated in the MHA plates, and a known quantity of 30C, 200C, and 1M of sepia officinalis and tellurium metallicum was applied to the discs using a micropipette. The plates were left at room temperature to allow sample absorption into the medium and were incubated at room temperature for 24 to 48 hours. The antifungal activity was determined by measuring the diameter of the inhibition zone. Fluconazole served as the positive control, while ethanol was the negative control.

In this study, Sepia officinalis 30C, 200C, 1M and Tellurium metallicum 30C, 200C, 1M did not exhibit any inhibitory activity against Trichophyton rubrum fungi. This experiment clearly indicated that the dilutions of both Sepia officinalis and Tellurium metallicum did not produce any inhibition zone.